Research Article Open Access

Induction of the Chemokines CCL3α, CCL3α and CCL5 in Atherosclerotic Patients

Alyaa Mousa1, Amani Al-Zaki2, Safa Taha3 and Moiz Bakhiet4
  • 1 Kuwait University, United Kingdom
  • 2 Genetics and Inherited Disorders Arabian Gulf University, United States
  • 3 Genetics and Inherited Disorders Arabian Gulf University,
  • 4 Genetics and Inherited Disorders Arabian Gulf University, United Kingdom

Abstract

Chemokines recruit immune cells to inflammatory sites and promote the process of inflammation. The role of these mediators in the disease process in atherosclerosis is not fully studied. The spontaneous mRNA expression and intracellular protein production of the potential inflammatory chemokines CCL3α and CCL3β (macrophage- inflammatory protein-1α and β; CCR3α/β ligand) and CCL5 (regulated upon activation, normal T cell expressed and secreted (RANTES; CCR5 ligand) in atherosclerotic patients was examined together with the effects of the chlamydial antigen HSP60 and LPS on the gene expression and protein induction of these mediators. Detection of chemokine mRNA and protein levels was assessed by in situ hybridization and immunohistochemistry respectively. The examined chemokines were detected at significantly high levels on atherosclerotic patients compared to healthy controls at both mRNA and protein levels. Stimulation with HSP60 and LPS from Chlamydia pneumoniae (C. pneumoniae) and E. coli showed increased numbers of CCL3α, CCL3β and CCL5 mRNA expressing cells in patients compared to health controls. Protein translation of these chemokines was depicted in correspondence to the mRNA gene expression for all examined chemokines spontaneously and after stimulation with chlamydial HSP60 and LPS and E. coli LPS. Thus, the herein data demonstrate the induction of potential inflammatory chemokines in atherosclerotic patients and that bacterial antigens play a role in the immunopathologic events in this disease by generating more inflammatory mediators.

American Journal of Immunology
Volume 3 No. 2, 2007, 45-51

DOI: https://doi.org/10.3844/ajisp.2007.45.51

Submitted On: 18 September 2007 Published On: 31 December 2007

How to Cite: Mousa, A., Al-Zaki, A., Taha, S. & Bakhiet, M. (2007). Induction of the Chemokines CCL3α, CCL3α and CCL5 in Atherosclerotic Patients. American Journal of Immunology, 3(2), 45-51. https://doi.org/10.3844/ajisp.2007.45.51

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Keywords

  • Atheroma
  • inflammation
  • mRNA
  • Chlamydia pneumoniae
  • E. coli