Research Article Open Access

Anti-Inflammatory Activity of Vitex leucoxylon L. Bark Extracts Against Freund's Complete Adjuvant Induced Arthritis in Sprague Dawley Rat

Alluri V. Krishnaraju1, Chundi B.M. Rao1, Dodda Sundararaju1, Krishanu Sengupta1 and Golakoti Trimurtulu1
  • 1 Laila Impex R and D Centre, India

Abstract

Problem statement: Anti-inflammatory agents, particularly steroids and cyclooxygenase inhibitors are often associated with adverse side effects including, GI irritation, ulcers, hypertension and cardiac abnormalities. A safe and effective intervention is essential for the treatment of inflammatory disorders. Approach: Abstract Vitex leucoxylon L., a medicinal plant of the verbenaceae family, used in traditional medicine for relieving headache and catarrh. VL-89/185A, was obtained by bio activity-guided fractionation using 5-Lipoxygenase inhibitory activity. VL-89/185A was further tested against Freund's Complete Adjuvant (FCA) induced arthritis in Sprague Dawley (SD) rat. Results: Oral supplementation of VL-89/185A resulted in significant anti-inflammatory effects as indicated by reduction in paw edema both at 100 and 250 mg kg-1 doses when compared to untreated control rats. Furthermore, treatment with VL-89/185A significantly reduced circulating proinflammatory cytokines TNF-α and IL-1β. The safety of VL-89/185A was established (LD50 >5000 mg kg-1 body) by acute oral toxicity limit test according to OECD guidelines 425. Conclusion: The safety and efficacy profiles indicated that VL-89/185A is a safe intervention for inflammatory disorders.

American Journal of Infectious Diseases
Volume 5 No. 2, 2009, 68-73

DOI: https://doi.org/10.3844/ajidsp.2009.68.73

Submitted On: 3 March 2009 Published On: 30 June 2009

How to Cite: Krishnaraju, A. V., Rao, C. B., Sundararaju, D., Sengupta, K. & Trimurtulu, G. (2009). Anti-Inflammatory Activity of Vitex leucoxylon L. Bark Extracts Against Freund's Complete Adjuvant Induced Arthritis in Sprague Dawley Rat. American Journal of Infectious Diseases, 5(2), 68-73. https://doi.org/10.3844/ajidsp.2009.68.73

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Keywords

  • Arthritis
  • IL-1β
  • 5-lipoxygenase
  • TNF-α
  • Vitex leucoxylon