Research Article Open Access

Molecular Characterization of Opium Poppy (Papaver somniferum) Germplasm

Hari Shanker Acharya1 and Vimal Sharma1
  • 1 Rajasthan College of Agriculture, India

Abstract

Problem statement: The medicinal value of opium poppy (2n = 22, Papaver somniferum L.) is due to presence of more than two dozen alkaloids. The genetic origin and diversity of cultivars used in the breeding collection is not fully known. To evaluate the genetic diversity of the 24 germplasm currently cultivated in the state to provide genetic information about heterogeneity, using RAPD and ISSR markers. Approach: Isolated genomic DNA was subjected to PCR amplification using either random or ISSR markers. The amplified fragments were scored and matrix was prepared. Jaccard similarity coefficients were calculated and dendogram was generated. Results: RAPD with 12 primers gave 46 scorable fragments out of which 32 bands were polymorphic (69.52%) with 2.6 polymorphic band per primer, while in ISSR analysis 9 primer gave good amplification with DNA of 24 opium poppy germplasm. They gave 27 scorable polymorphic with 3.0 bends per primer. Dendrogram constructed revealed that the based on RAPD the germplasm cultivated comprised of one major group A and one minor group B, while two clusters of two germplasm each stands apart, on the basis of ISSR they fall into one major group A and one minor groups B, while one germplasm clusters of UOP-60 stands apart. Based on combined RAPD and ISSR analysis these germplasm were divided into 1 major group A and 3 minor group B, C, D whereas one genotype UOP-6 stood apart. Conclusion: These results showed that the majority of cultivated germplasm fall in one cluster and have low genetic diversity.

American Journal of Infectious Diseases
Volume 5 No. 2, 2009, 148-153

DOI: https://doi.org/10.3844/ajidsp.2009.148.153

Submitted On: 20 March 2009 Published On: 30 June 2009

How to Cite: Acharya, H. S. & Sharma, V. (2009). Molecular Characterization of Opium Poppy (Papaver somniferum) Germplasm. American Journal of Infectious Diseases, 5(2), 148-153. https://doi.org/10.3844/ajidsp.2009.148.153

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Keywords

  • RAPD
  • ISSR
  • molecular markers