Effect of Verapamil on Serum Level of Salinomycin in Diabetic Rats
- 1 Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
- 2 Department of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
Abstract
Problem statement: The aim of present study was evaluation function of P-glycoprotein with or without verapamil in normal and diabetic rats; which the function of P-glycoprotein was indirectly evaluated by detection of serum slainomycin concentration with HPLC method. Approach: This study was carried in 4 groups of rats including normal rats which received salinomycin and verapamil together salinomycin; and diabetic rats which received salinomycin and verapamil together salinomycin. Serum concentration of salinomycin was measured by HPLC after 3 h from its administration. Results: Results show the serum concentration of salinomycin significantly elevated in diabetic rats which received verapamil together salinomycin; while this concentration did not significantly change in other groups. Conclusion: Since the p-glycoprotein activity decreases in diabetic conditions and verapamil inhibits it; probably transport of salinomycin from blood to tissues or its elimination was decreased that caused its elevated serum concentration.
DOI: https://doi.org/10.3844/ajassp.2011.860.863
Copyright: © 2011 Hossein Najafzadeh, Said Reza Fatemi and Amir Ashna. This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Salinomycin significantly
- verapamil inhibits
- diabetes induction
- HPLC method
- serum concentration
- p-glycoprotein
- diabetic rats
- serum level
- tumor cells